Part:BBa_K4574007

Anti-toxin A IGVH

Variable heavy chain locus of the human AR-301 monoclonal IgG1 antibody, which specifically targets toxin A (hemolysin) of Staphylococcus aureus. This component is integral to tosatoxumab, a therapeutic antibody used against infections caused by MRSA.

Source

The following basic part was developed for the therapeutic monoclonal antibody against S. aureus toxin A by Rudolf et al.^[1].

The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.

Usage and Biology

This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 169

References

↑ Rudolf, M., & Koch, H. (2011). Human monoclonal antibody against S. aureus derived alpha-toxin and its use in treating or preventing abscess formation (World Intellectual Property Organization Patent WO2011018208A1). https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2011018208

[1] Rudolf, M., & Koch, H. (2011). Human monoclonal antibody against S. aureus derived alpha-toxin and its use in treating or preventing abscess formation (World Intellectual Property Organization Patent WO2011018208A1). https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2011018208

[1]